$495.00
Cat. #: 26160 |
Gene Symbol: IDH1; IDCD; IDH; IDP; IDPC; PICD |
Description: Anti-IDH1(R132S) Mouse Monoclonal Antibody |
Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
Immunogen: A synthetic peptide from the internal region of IDH1 which includes the mutation of R132S, human origin. |
Applications:ELISA, WB, IF, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes IDH1(R132S) mutated protein, but not wild type IDH1 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
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