NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
IDH2(R172K)
Cat. #: 26163 |
Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
Description: Anti-IDH2(R172K) Mouse Monoclonal Antibody |
Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas. |
Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 IHC: 1:10-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: recognizes R172K mutant, but not wild type IDH2 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant IDH2(R172K) and wild type proteins.
Purified His-tagged IDH2(R172K) (lane 2) and wild type protein (lane 1) were blotted with Anti-IDH2(R172K) monoclonal antibody (Cat. #26163).
Immunofluorescence:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172K) antibody.
HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #26163).