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Human LGR4 Full Length Protein

Human LGR4 Full Length Protein-Synthetic Nanodisc


Cat.#:  11053

   Size:   50 μg

In Stock

          Product Description          
Cat.#:     11053
Product Name:   Human LGR4 Full Length Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   BNMD17; GPR48
Target:    LGR4
Description:    Human LGR4 full length protein-synthetic nanodisc
Background:     Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye.
Species/Host:    HEK293
Molecular Weight:    The human full length LGR4 protein has a MW of 104.5 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag LGR4-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with LGR4-Nanodisc is 5.224ng/ml.

Figure2. Human LGR4-Nanodisc, Flag Tag on SDS-PAGE