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mesothelin (DM71) Rabbit mAb

Anti-mesothelin (DM71) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28383

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28383
Product Name:   Anti-Mesothelin(DM71) Rabbit Monoclonal Antibody
Synonyms:   MSLN; Mesothelin; MPF
Description:    Anti-mesothelin antibody(DM71) Rabbit Monoclonal Antibody
Background:    This gene encodes a preproprotein that is proteolytically processed to generate two protein products; megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas; ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants; at least one of which encodes an isoform that is proteolytically processed.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human Mesothelin
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human MSLN protein, mFc-His tagged protein 11159 can bind Rabbit anti-MSLN monoclonal antibody ( clone: DM71) in a linear range of 1-100 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein  (A) and human mesothelin  (B) were surface stained with Rabbit anti-MSLN monoclonal antibody 1µg/ml ( clone: DM71) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM71) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 4. Affinity ranking of different Rabbit anti-MSLN mAb clones by titration of different concentration onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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