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PIK3CA(D549N)

Anti-PIK3CA(D549N) Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26215

   Size:   100 μl

In Stock

          Product Description          
PIK3CA(D549N)
Cat. #:  26215
Size:  100 µL
Gene Symbol:  PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
Description:   Anti-PIK3CA(D549N) Mouse Monoclonal Antibody
Background:   Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
Immunogen:   A synthetic peptide from the internal region of PIK3CA which includes the mutation of D549N, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100
Concentration:   0.5 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D549N mutant, but not wild type PIK3CA of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant PIK3CA(D549N) and wild type proteins. Purified His-tagged PIK3CA(D549N) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(D549N) monoclonal antibody (Cat. #26215).
Immunofluorescence:   Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(D549N) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(D549N) plasmid (right column), then fixed and stained with Anti-PIK3CA(D549N) monoclonal antibody (Cat. #26215).
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