$495.00
| Anti-Rab35GTP Monoclonal Antibody |
| Cat. #: 26922 |
| Size: 30 µL |
| Gene Symbol: RAB35 |
| Description: Anti-Rab35-GTP Monoclonal Antibody |
| Background: The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions. |
| Immunogen: Recombinant full length protein of active Rab35 |
| Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Rab35 GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-Rab35-GTP monoclonal antibody recognizes active Rab35 proteins of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
Rab35 Activation Assay.
Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-Rab35-GTP monoclonal antibody (Cat. No. 26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. 21078). |
| 1. IL-17A Recruits Rab35 to IL-17R to Mediate PKCα-Dependent Stress Fiber Formation and Airway Smooth Muscle Contractility J Immunol. 2019 2019 PMID: 30683702 |
| 2. Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway J Neurosci. 2016 PMID: 27535913 |
| 3. Molecular Mechanisms of Synaptic Vesicle Degradation academiccommons.columbia. 2016 PMID: |
| 4. Calcium dependent regulation of Rab activation and vesicle fusion by an intracellular P2X ion channel Nat Cell Biol. 2014 PMID: 24335649 |
