Pioneering GTPase and Oncogene Product Development since 2010

Email us:

Call us: 6109452007

Rab3A(Q81L) Protein

Rab3A Protein Q81L mutant


Cat.#:  10129

   Size:   25 μl

In Stock

            Product Description          

Rab3A(Q81L) Mutant

Cat. #:  10129
Product Name:  Rab3A Protein Q81L mutant
Synonyms:  Member RAS oncogene family
Source:   Human, recombinant full length, His6-tag
Expression Host Species:   E. coli
Molecular Weight:   25 kDa
Purity:   >95% by SDS-PAGE
Introduction:   The Rab3 small G protein family consists of four members, Rab3A, -3B, -3C, and -3D. Rab3A is found specifically in brain and regulates a late step in synaptic vesicle fusion. Rab3A -mediated synaptic transmission is involved in circadian period and sleep homeostasis.
Amino Acid Sequence   (1-220, Q81L)
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear
Concentration: 1 mg/mL
Storage:   -80°C
Preparation Instructions:  
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab3A Q81L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.

1. Geppert, M. et al., Nature 387: 810-814, 1997.
2. Giovedi, S. et al., J. Biol. Chem. 279: 43769-43779, 2004.
3. Huang, Y.-Y. et al., Proc. Nat. Acad. Sci. 102: 9365-9370, 2005.
4. Kapfhamer, D. et al., Nature Genet. 32: 290-295, 2002.
5. Rousseau-Merck, M. F. et al., Genomics 5: 694-698, 1989.
6. Rousseau-Merck, M. F. et al., Cytogenet. Cell Genet. 51: 1070-only, 1989.
7. Sullivan, M. et al., Cell. Signal. 11: 735-742, 1999.
8. Trask, B. et al., Genomics 15: 133-145, 1993.
9. Zahraoui, A. et al., J. Biol. Chem. 264: 12394-12401, 1989.