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Rab3A(Q81L) Protein

Rab3A Protein Q81L mutant
5/5

$347.00

Cat.#:  10129

   Size:   25 μl

In Stock

            Product Description          

Rab3A(Q81L) Mutant

Cat. #:  10129
Product Name:  Rab3A Protein Q81L mutant
Synonyms:  Member RAS oncogene family
Source:   Human, recombinant full length, His6-tag
Expression Host Species:   E. coli
Molecular Weight:   25 kDa
Purity:   >95% by SDS-PAGE
Introduction:   The Rab3 small G protein family consists of four members, Rab3A, -3B, -3C, and -3D. Rab3A is found specifically in brain and regulates a late step in synaptic vesicle fusion. Rab3A -mediated synaptic transmission is involved in circadian period and sleep homeostasis.
Amino Acid Sequence   (1-220, Q81L)
MASATDSRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRN
DKRIKLQIWDTAGLERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVG
NKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTGAKQ
GPQLSDQQVPPHQDCAC
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear
Concentration: 1 mg/mL
Storage:   -80°C
Preparation Instructions:  
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab3A Q81L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.


References:  
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