NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$219.00
Rac1 Polyclonal Antibody
Cat. #: 21003 |
Gene Symbol: RAC1 |
Description: Anti-Rac1 Rabbit Polyclonal Antibody |
Background: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
Immunogen: A synthetic peptide from a unique region of Rac1 |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA 1:100-1:5000, WB 1:100-1:2000 |
Concentration: 1 mg/ml |
Host Species: Rabbit |
Format: Liquid |
Clonality: Polyclonal |
Isotype: IgG |
Purity: Purified from serum |
Preservative: No |
Constituents: PBS ((without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Rac1 antibody recognizes Rac1 from vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of Rac1 proteins. MEF whole-cell lysate was used for western blots with the polyclonal anti Rac1 antibody (cat # 21003). |
1. Voltage-dependent activation of Rac1 by Nav1.5 channels promotes cell migration J Cell Physiol. 2020 PMID:31612502 Application: IHC |
2. The modulation of MiR-155 and MiR-23a manipulates Klebsiella pneumoniae Adhesion on Human pulmonary Epithelial cells via Integrin a5ß1 Signaling Sci Rep. 2016 PMID:27534887 Application: IP |
3. The cooperative action of bacterial fibronectin-binding proteins and secreted proteins promote maximal Campylobacter jejuni invasion of host cells by stimulating membrane ruffling Cell Microbiol. 2012 PMID:21999233 Application: IP |