NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Cat. #: 26479 |
Gene Symbol: H-Ras; K-Ras; N-Ras |
Description: Anti-RAS(A146V) Mouse Monoclonal Antibody |
Background: The A146V mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146V mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. |
Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of A146V, human origin. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes A146V mutant, but not wild type RAS of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of recombinant RAS(A146V) and wild type proteins. Purified His-tagged RAS(A146V) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-RAS(A146V) monoclonal antibody (Cat. #26479). Immunofluorescence: Immunofluorescence of cells expressing KRas proteins with anti-KRAS(A146V) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRAS(A146V) plasmid, pCDNA3-GFP-KRas (WT) plasmid, pCDNA3-GFP-KRas (A146P) plasmid or pCDNA3-GFP-KRas (A146T) plasmid, then fixed and stained with anti-KRAS(A146V) monoclonal antibody (Cat. #26479). |