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CTLA4 mAb (Reference mAb)

Anti-CTLA4 Monoclonal Antibody (Reference mab)
5/5

$199.00

Cat.#:  28031

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28031
Product Name:   Anti-CTLA4 Reference Monoclonal Antibody
Synonyms:   CTLA4;CD152
Description:    Anti-CTLA4 Reference Monoclonal Antibody
Background:    Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Homo sapiens
Isotype:    IgG1
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human CTLA-4
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA4, mFc-His tagged protein 11145 can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.13-16.0 ng/ml.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA4, hFc tagged protein (11289) can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.64-80.0 ng/ml. In order to specifically detect 28031, mouse anti-human Fab-specific antibody was used as detection antibody.

Figure 3. HEK293 cell line transfected with irrelevant protein  (B) and human CTLA4  (A) were surface stained with anti-CTLA4 neutralizing antibody 1µg/ml (ipilimumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.
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