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RhoA Pull-Down Activation Assay Kit

RhoA Pull-Down Assay Kit
5/5

$737.00

Cat.#:  80601

   Size:   30 Assays

In Stock

          Product Description          

RhoA Pull-Down Activation Assay Kit

Cat. # 80601

Introduction

A. Background
Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of RhoA, and the hydrolysis of GTP to GDP renders it inactive.
Currently the activation of RhoA proteins is assayed with the binding of GTP-bound RhoA to the p21-binding domain (PBD) of p21-activated protein kinase (PAK). This method is based on the observation that the active, GTP-bound RhoA could bind to the PBD of PAK. However, the reproducibility of this method is poor. This is partially due to the relatively quick hydrolysis of GTP to GDP during the assay procedure, and the low binding affinity of PBD to RhoA-GTP.
The RhoA Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes RhoA-GTP, but not RhoA-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a much shorter time. This assay provides the reliable results with consistent reproducibility.
The anti-RhoA-GTP monoclonal antibody can also be used to monitor the activation of RhoA in cells and in tissues by immunohistochemistry.
B. Assay Principle
The RhoA Activation Assay Kit uses configuration-specific anti-RhoA-GTP Mouse monoclonal antibody to measure RhoA-GTP levels in cell extracts or in vitro GTPγS loading RhoA activation assays. Anti-RhoA-GTP mouse monoclonal antibody is first incubated with cell lysates containing RhoA-GTP. Next, the GTP-bound RhoA is pulled down by protein A/G agarose. Finally, the precipitated RhoA-GTP is detected through immunoblot analysis using Anti-RhoA Rabbit Polyclonal Antibody.
C. Kit Components
1. Anti-RhoA-GTP Mouse Monoclonal Antibody (Cat. # 26904): 30 µL (1 mg/ml) in PBS, pH 7.4, containing 50% glycerol. This antibody specifically recognizes RhoA-GTP from all vertebrates.
2. Protein A/G Agarose (Cat. # 30301): 600 µL of 50% slurry.
3. 5X Assay/Lysis Buffer (Cat. # 30302): 30 mL of 250 mM Tris-HCl, pH 8, 750 mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti-RhoA Rabbit Polyclonal Antibody (Cat. # 21017): 50 µL (1 mg/mL) in PBS, pH 7.4, contained 50% glycerol.
5. 100X GTPγS (Cat. # 30303): 50 µl at 10 mM, use 5 µL of GTPγS for  GTP-labeling of 0.5 mL of cell lysate.
6. 100X GDP (Cat. # 30304): 50 µl at 100 mM, use 5 µL of GDP for GDP-labeling of 0.5 mL of cell lysate.
7. HRP-Goat Anti-Rabbit IgG (Cat. # 29002): 50 µL (0.4 µg/mL) in PBS, pH 7.4, contained 50% glycerol.
D. Materials Needed but Not Supplied
1. Stimulated and non-stimulated cell lysates
2. Protease inhibitors
3. 4 °C tube rocker or shaker
4. 0.5 M EDTA at pH 8.0
5. 1.0 M MgCl2
6. 2X reducing SDS-PAGE sample buffer
7. Electrophoresis and immunoblotting systems
8. Immunoblotting wash buffer such as TBST (10 mM Tris-HCl, pH 7.4, 0.15 M NaCl, 0.05%  Tween-20)
9. Immunoblotting blocking buffer (TBST containing 5% Non-fat Dry Milk or 3% BSA)
10. ECL Detection Reagents
E. Example Results
The following figure demonstrates example results seen with the RhoA Activation Assay Kit. For reference only.
RhoA Activation Assay Kit RhoA Activation Assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-RhoA-GTP monoclonal antibody (Cat. # 26904) (top panel). The precipitated RhoA-GTP was immunoblotted with an anti-RhoA rabbit polyclonal antibody (Cat # 21017). The bottom panel shows the Western blot with anti-RhoA of the cell lysates used (5% of that used in the top panel).

Assay Procedure

A. Reagent Preparation
1X Assay/Lysis Buffer: Mix the 5X Stock (Cat. # 30302) briefly and dilute with deionized water to make 1X buffer. Just prior to usage, add protease inhibitors such as 1 mM PMSF, 10 µg/mL leupeptin, and 10 µg/mL aprotinin.
B. Sample Preparation
Adherent Cells
1. Culture cells (one 10-cm plate, ~107 cells) to approximately 80-90% confluence. Stimulate the cells with activator or inhibitor as desired.
2. Aspirate the culture media and wash twice with ice-cold PBS.
3. Completely remove the final PBS wash and add ice-cold 1X Assay/Lysis Buffer (See Reagent Preparation) to the cells (0.5-1 mL per 10 cm tissue culture plate).
4. Place the culture plates on ice for 10-20 minutes.
5. Detach the cells from the plates by scraping with a cell scraper.
6. Transfer the lysates to appropriate size tubes and place on ice.
7. If nuclear lysis occurs, the cell lysates may become viscous and difficult to pipette. If this occurs, lysates can be passed through a 27½-gauge syringe needle 3-4 times to shear the genomic DNA.
8. Clear the lysates by centrifuging at 12,000 x g and 4°C for 10 minutes.
9. Collect the supernatant and store the sample (~1-2 mg of total protein) on ice for immediate use, or snap freeze and store at -70°C for future use.
Suspension Cells
1. Culture cells and stimulate with activator or inhibitor as desired.
2. Perform a cell count and then pellet the cells through centrifugation.
3. Aspirate the culture media and wash twice with ice-cold PBS.
4. Completely remove the final PBS wash and add ice-cold 1X Assay/Lysis Buffer (See Reagent Preparation) to the cell pellet (0.5-1 mL per 107 cells).
5. Lyse the cells by repeated pipetting.
6. Transfer the lysates to appropriate size tubes and place them on ice.
7. If nuclear lysis occurs, the cell lysates may become viscous and difficult to pipette. If this occurs, lysates can be passed through a 27½-gauge syringe needle 3-4 times to shear the genomic DNA.
8. Clear the lysates by centrifuging at 12,000 x g and 4°C for 10 minutes.
9. Collect the supernatant and store sample on ice for immediate use, or snap freeze and store at -70°C for future use.
C. In vitro GTPγS/GDP Protein for Positive and Negative controls
Note: In vivo stimulation of cells will activate approximately 10% of the available RhoA, whereas in vitro GTPγS protein loading will activate nearly 90% of RhoA.
1. Aliquot 0.5 mL of cell extract (or 1 µg of purified RhoA protein) into two microcentrifuge tubes.
2. To each tube, add 20 µL of 0.5 M EDTA (final concentration of 20 mM).
3. Add 5 µL of 100 X GTPγS (Cat. # 30303) to the first tube as a positive control.
4. Add 5 µL of 100 X GDP (Cat. # 30304) to the second tube as a negative control.
5. Incubate both tubes at 30°C for 30 minutes with agitation.
6. Stop loading by placing the tubes on ice and adding 32.5 µL of 1 M MgCl2 (final concentration of 60 mM).
D. Affinity Precipitation of Activated G Protein
1. Aliquot 0.5-1 mL of cell lysates (about 1 mg of total cellular protein) to a microcentrifuge tube.
2. Adjust the volume to 1 mL with 1X Assay/Lysis Buffer (See Reagent Preparation).
3. Add 1 µL anti-RhoA-GTP antibody (Cat. # 26904).
4. Prepare the protein A/G Agarose bead slurry (Cat. # 30301) by resuspending through vertexing or titrating.
5. Quickly add 20 µL of resuspended bead slurry to above tube.
6. Incubate the tube at 4°C for 1 hour with gentle agitation.
7. Pellet the beads through centrifugation at 5,000 x g for 1 min.
8. Aspirate and discard the supernatant (making sure not to disturb or remove the bead pellet).
9. Wash the beads 3 times with 0.5 mL of 1X Assay/Lysis Buffer, centrifuging and aspirating each time.
10. After the third wash, pellet the beads through centrifugation and carefully remove all the supernatant.
11. Resuspend the bead pellet in 20 µL of 2X reducing SDS- PAGE sample buffer.
12. Boil the sample for 5 minutes.
13. Centrifuge it at 5,000 x g for 10 seconds.
E. Western Blot Analysis
1. Load 15 µL/well of pull-down supernatant to a polyacrylamide gel (17%). It is recommended to include a pre-stained MW standard (as an indicator of a successful transfer in step 3 below).
2. Perform SDS-PAGE following the manufacturer’s instructions.
3. Transfer the gel proteins to a PVDF or nitrocellulose membrane following the manufacturer’s instructions.
Note: Steps 4-11 are at room temperature with agitation
4. Following electroblotting, immerse the PVDF membrane in 100% Methanol for 15 seconds, and then allow it to dry at room temperature for 5 minutes.
Note: If Nitrocellulose is used instead of PVDF, step 4 Should be skipped.
5. Block the membrane with 5% non-fat dry milk or 3% BSA in TBST for 1 hr at room temperature with constant agitation.
6. Wash the blotted membrane three times with TBST, 5 minutes each time.
7. Incubate the membrane with Anti-RhoA Rabbit Polyclonal Antibody (Cat. # 21017), which is freshly diluted 1:50~500 (depending on the amount of RhoA proteins in your sample) in 5% non-fat dry milk or 3% BSA in TBST, for 1-2 hr at room temperature with constant agitation or at 4°C overnight.
8. Wash the blotted membrane three times with TBST, 5 minutes each time.
9. Incubate the membrane with a secondary antibody (Cat. # 29002), which is freshly diluted 1:1000 in 5% non-fat dry milk or 3% BSA in TBST, for 1 hr at room temperature with constant agitation.
10. Wash the blotted membrane three times with TBST, 5 minutes each time.
11. Use the detection method of your choice such as ECL.
          Publications          
001. Baihui (DU20)-penetrating Qubin (GB7) acupuncture on blood-brain barrier integrity in rat intracerebral hemorrhage models via the RhoA/ROCK II/MLC 2 signaling pathway
Animal Model Exp Med. 2024 PMID:38379356
002. FBXL8 inhibits post-myocardial infarction cardiac fibrosis by targeting Snail1 for ubiquitin-proteasome degradation
Cell Death Dis. 2024 PMID:38615011
003. Infiltrative vessel co-optive growth pattern induced by IQGAP3 overexpression promotes microvascular invasion in hepatocellular carcinoma
Clin Cancer Res. 2024 PMID:38470497
004. Sustainable inflammatory activation following spinal cord injury is driven by thrombin-mediated dynamic expression of astrocytic chemokines
Brain Behav Immun. 2024 PMID:38042209
005. Balancing act of small GTPases downstream of plexin-A4 signaling motifs promotes dendrite elaboration in mammalian cortical neurons
Sci Signal. 2024 PMID:38227686
006. Tensin-3 is involved in osteogenic versus adipogenic fate of human bone marrow stromal cells
Cell Mol Life Sci. 2023 PMID:37668682
007. Tumoricidal Activity of Simvastatin in Synergy with RhoA Inactivation in Antimigration of Clear Cell Renal Cell Carcinoma Cells
Int J Mol Sci. 2023 PMID:37298689
008. Glucuronoxylomannan intranasal challenge prior to Cryptococcus neoformans pulmonary infection enhances cerebral cryptococcosis in rodents
PLoS Pathog. 2023 PMID:37115795
009. Nogo-A is secreted in extracellular vesicles, occurs in blood and can influence vascular permeability
J Cereb Blood Flow Metab. 2023 PMID:38000040
010. Targeting ASIC1a Promotes Neural Progenitor Cell Migration and Neurogenesis in Ischemic Stroke
Research (Wash D C). 2023 PMID:37275123
011. Rac1 and Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneurons
J Cell Sci. 2023 PMID:36744839
012. Mechanosensitive brain tumor cells construct blood-tumor barrier to mask chemosensitivity
Neuron. 2023 PMID:36323321
013. The activated CD36-Src axis promotes lung adenocarcinoma cell proliferation and actin remodeling-involved metastasis in high-fat environment
Cell Death Dis. 2023. 2023 PMID:37612265
014. Ogt Deficiency Induces Abnormal Cerebellar Function and Behavioral Deficits of Adult Mice through Modulating RhoA/ROCK Signaling
J Neurosci. 2023 PMID:37225434
015. Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasis
J Cell Commun Signal. 2023 PMID: 36477766
016. Reducing GEF-H1 Expression Inhibits Renal Cyst Formation, Inflammation, and Fibrosis via RhoA Signaling in Nephronophthisis
Int J Mol Sci. 2023 PMID: 36834937
017. Junctional Force Patterning drives both Positional and Orientational Order in Auditory Epithelia
Research Square. 2023 PMID:
018. CD16+ fibroblasts foster a trastuzumab-refractory microenvironment that is reversed by VAV2 inhibition
Cancer Cell. 2022 PMID:36379207
019. BKM120 alters the migration of doublecortin-positive cells in the dentate gyrus of mice
Pharmacol Res. 2022 PMID:35460881
020. Liquiritigenin alleviates doxorubicin-induced chronic heart failure via promoting ARHGAP18 and suppressing RhoA/ROCK1 pathway
Exp Cell Res. 2022 PMID:34990617
021. Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasis
J Cell Commun Signal. 2022 PMID:36284029
022. Cystic fibrosis macrophage function and clinical outcomes after elexacaftor/tezacaftor/ivacaftor
Eur Respir J. 2022 PMID:36265882
023. ARHGEF15 in Sertoli cells contributes to germ cell development and testicular immune privilege
Biol Reprod. 2022 PMID:36001358
024. Rac1 mediates cadherin-11 induced cellular pathogenic processes in aortic valve calcification
Cardiovasc Pathol. 2022 PMID:35074515
025. Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activation
Cardiovasc Res. 2022 PMID:34528082
026. IGF2BP2 promotes gastric cancer progression by regulating the IGF1R-RhoA-ROCK signaling pathway
Cell Signal. 2022 PMID:35306138
027. Long-term exposure to low concentrations of MC-LR induces blood-testis barrier damage through the RhoA/ROCK pathway
Ecotoxicol Environ Saf. 2022 PMID:35367887
028. ARHGEF37 overexpression promotes extravasation and metastasis of hepatocellular carcinoma via directly activating Cdc42
J Exp Clin Cancer Res. 2022 PMID:35869555
029. Hypoxia-activated neuropeptide Y/Y5 receptor/RhoA pathway triggers chromosomal instability and bone metastasis in Ewing sarcoma
Nat Commun. 2022 PMID:35484119
030. Modulation of Mechanical Stress Mitigates Anti-Dsg3 Antibody-Induced Dissociation of Cell-Cell Adhesion
Adv Biol (Weinh). 2021 PMID:33724731
031. MYO1B enhances colorectal cancer metastasis by promoting the F-actin rearrangement and focal adhesion assembly via RhoA/ROCK/FAK signaling
Ann Transl Med. 2021 PMID:34790749
032. Glucocorticoid-mediated induction of caveolin-1 disrupts cytoskeletal organization.inhibits cell migration and re-epithelialization of non-healing wounds
Commun Biol. 2021 PMID:34145387
033. Hydrostatic mechanical stress regulates growth and maturation of the atrioventricular valve
Development. 2021 PMID:34086041
034. Oxygen regulates epithelial stem cell proliferation via RhoA-actomyosin-YAP/TAZ signal in mouse incisor
Development. 2021 PMID: 33472844
035. ROCK inhibitor attenuates carbon blacks-induced pulmonary fibrosis in mice via Rho/ROCK/NF-kappa B pathway
Environ Toxicol. 2021 PMID:33792148
036. BBS7–SHH Signaling Activity Regulates Primary Cilia for Periodontal Homeostasis
Front Cell Dev Biol. 2021 PMID:34957122
037. Benzo(a)pyrene Enhanced Dermatophagoides Group 1 (Der f 1)-Induced TGFß1 Signaling Activation Through the Aryl Hydrocarbon Receptor-RhoA Axis in Asthma
Front Immunol. 2021 PMID:33936062
038. Activin A and Cell-Surface GRP78 Are Novel Targetable RhoA Activators for Diabetic Kidney Disease
Int J Mol Sci. 2021 PMID:33799579
039. Modulation of proteoglycan receptor regulates RhoA/CRMP2 pathways and promotes axonal myelination
Neurosci Lett. 2021 PMID:34166723
040. Modulation of proteoglycan receptor regulates RhoA/CRMP2 pathways and promotes axonal myelination
Neurosci Lett. 2021 PMID:34166723
041. Decreased Level of Exosomal miR-5121 Released from Microglia Suppresses Neurite Outgrowth and Synapse Recovery of Neurons Following Traumatic Brain Injury
Neurotherapeutics. 2021 PMID:33475953
042. The role of daurisoline treatment in hepatocellular carcinoma: Inhibiting vasculogenic mimicry formation and enhancing sensitivity to sorafenib
Phytomedicine. 2021 PMID:34600176
043. MYBL2 disrupts the Hippo-YAP pathway and confers castration resistance and metastatic potential in prostate cancer
Theranostics. 2021 PMID:33897882
044. Crosstalk Between Abnormal TSHR Signaling Activation and PTEN/PI3K in the Dedifferentiation of Thyroid Cancer Cells
Front Oncol. 2021 PMID:34650915
045. Astrocytes close the critical period for visual plasticity
bioRxiv. 2020 PMID:34210880
046. ANLN Directly Interacts with RhoA to Promote Doxorubicin Resistance in Breast Cancer Cells
Cancer Manag Res. 2020 PMID:33116832
047. Inhibition of TRPM7 blocks MRTF/SRF-dependent transcriptional and tumorigenic activity
Oncogene. 2020 PMID:31844251
048. Microglia Dysfunction Caused by the Loss of Rhoa Disrupts Neuronal Physiology and Leads to Neurodegeneration
Cell Rep. 2020 PMID:32579923
049. QUAKING Regulates Microexon Alternative Splicing of the Rho GTPase Pathway and Controls Microglia Homeostasis
Cell Rep. 2020 PMID:33378678
050. Serotonin 5-HT4 receptor boosts functional maturation of dendritic spines via RhoA-dependent control of F-actin
Commun Biol. 2020 PMID:32060357
051. Ligand Binding to the Collagen VI Receptor Triggers a Talin-to-RhoA Switch that Regulates Receptor Endocytosis
Dev Cell. 2020 PMID:32428455
052. europeptide Y/Y5 Receptor Pathway Stimulates Neuroblastoma Cell Motility Through RhoA Activation
Front Cell Dev Biol. 2020 PMID:33681186
053. Arhgef5 Binds a-Dystrobrevin 1 and Regulates Neuromuscular Junction Integrity
Front Mol Neurosci. 2020 PMID:32587503
054. Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafishs
Nat Commun. 2020 PMID:32620775
055. Dysregulation of Rac or Rho elicits death of motor neurons and activation of these GTPases is altered in the G93A mutant hSOD1 mouse model of amyotrophic lateral sclerosis
Neurobiol Dis. 2020 PMID:31931138
056. Acute compressive stress activates RHO/ROCK-mediated cellular processes
Small GTPases. 2020 PMID:29455593
057. Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation
Stem Cells Int. 2020 PMID:32508931
058. Mitigative Effect of Sipjeondaebo-tang on RhoA Activation in Cold-Exposed Vascular Cells
The Journal of Internal Korean Medicine. 2020 PMID:https://www.jikm.or.kr/journal/view.php?doi=10. 22246/jikm. 2020.41.4.553
059. Matrine inhibits the development and progression of ovarian cancer by repressing cancer associated phosphorylation signaling pathways
Cell Death & Disease,2019 PMID:31601793
060. The alteration of RhoA geranylgeranylation and Ras farnesylation breaks the integrity of the blood–testis barrier and results in hypospermatogenesis
Cell Death Dis. 2019 PMID:31171774
061. BKCa compensates impaired coronary vasoreactivity through RhoA/ROCK pathway in hind-limb unweighted rats
FASEB J. 2019 PMID: 31530101
062. The role of sema4D in vasculogenic mimicry formation in non-small cell lung cancer and the underlying mechanisms
Int J Cancer. 2019 PMID: 30374974
063. RhoA/ROCK Signaling Modulates Lineage Commitment of Mesenchymal Stem Cells in Asthma through Lef1
J Allergy Clin Immunol. 2019 PMID:30194990
064. Claudin-2 suppresses GEF-H1.RHOA.and MRTF.thereby impacting proliferation and profibrotic phenotype of tubular cells
J Biol Chem. 2019 PMID:31481470
065. Sensory plasticity of carotid body is correlated with oxidative stress in paraventricular nucleus during chronic intermittent hypoxia
J Cell Physiol. 2019 PMID:30609027
066. AMPA receptors modulate the reorganization of F-actin in Bergmann glia cells through the activation of RhoA
J Neurochem. 2019 PMID:30589940
067. Inhibition of Notch1 signaling promotes neuronal differentiation and improves functional recovery in spinal cord injury through suppressing the activation of Ras homolog family member A
J Neurochem. 2019 PMID:31339573
068. miR-511-3p protects against cockroach allergen-induced lung inflammation by antagonizing CCL2
JCI Insight. 2019 PMID:31536479
069. Simvastatin pretreatment ameliorates t-PA-induced hemorrhage transformation and MMP-9/TIMP-1 imbalance in thromboembolic cerebral ischemic rats
Neuropsychiatr Dis Treat. 2019 PMID:31410004
070. P120-Catenin And Its Phosphorylation On Tyr228 Inhibits Proliferation And Invasion In Colon Adenocarcinoma Cells
Onco Targets Ther. 2019 PMID:32063714
071. Increased expression of GEF-H1 promotes colon cancer progression by RhoA signaling
Pathology – Research and Practice. 2019 PMID:30846413
072. 12(S)-Hydroxyeicosatetraenoic acid downregulates monocyte-derived macrophage efferocytosis: New insights in atherosclerosis
Pharmacol Res. 2019 PMID:31028904
073. Inhibition of Rac1-dependent forgetting alleviates memory deficits in animal models of Alzheimer’s disease
Protein Cell. 2019 PMID:31321704
074. Profibrotic epithelial phenotype: a central role for MRTF and TAZ
Sci Rep. 2019 PMID:30867502
075. Endothelial RhoA GTPase is essential for in vitro endothelial functions but dispensable for physiological in vivo angiogenesis
Scientific Reports. 2019 PMID:31406143
076. RhoA increases ASIC1a plasma membrane localization and calcium influx in pulmonary arterial smooth muscle cells following chronic hypoxia
Am J Physiol Cell Physiol. 2018 PMID:29070491
077. E3 Ubiquitin-Protein Ligase SMURF1 in the Nucleus Accumbens Mediates Cocaine Seeking
Biol Psychiatry. 2018 PMID:30158054
078. Naringin attenuates MLC phosphorylation and NF-?B activation to protect sepsis-induced intestinal injury via RhoA/ROCK pathway
Biomed Pharmacother. 2018 PMID:29635128
079. Inhibition of RhoA reduces propofol-mediated growth cone collapse.axonal transport impairment.loss of synaptic connectivity and behavioural deficits
Br J Anaesth. 2018 PMID:29576115
080. Geranylgeranyl pyrophosphate synthase facilitates the organization of cardiomyocytes during mid-gestation through modulating protein geranylgeranylation in mouse heart
Cardiovasc Res. 2018 PMID:29444209
081. The Rho GTPase Rnd1 inhibits epithelial–mesenchymal transition in hepatocellular carcinoma and is a favorable anti-metastasis target
Cell Death Dis. 2018 PMID:29706627
082. Social Isolation Induces Rac1-Dependent Forgetting of Social Memory
Cell Rep. 2018 PMID:30304669
083. Fam65b Phosphorylation Relieves Tonic RhoA Inhibition During T Cell Migration
Front Immunol. 2018 PMID:30254631
084. The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+ T cells
J Exp Med. 2018 PMID:29875261
085. Swedish Nerve Growth Factor Mutation (NGFR100W) Defines a Role for TrkA and p75NTR in Nociception
J Neurosci. 2018 PMID:29483280
086. COL1A1 promotes metastasis in colorectal cancer by regulating the WNT/PCP pathway
Mol Med Rep. 2018 PMID:29393423
087. ß-Elemene Enhances GAP-43 Expression and Neurite Outgrowth by Inhibiting RhoA Kinase Activation in Rats with Spinal Cord Injury
Neuroscience. 2018 PMID:29751054
088. MicroRNA-650 targets inhibitor of growth 4 to promote colorectal cancer progression via mitogen activated protein kinase signaling
Oncol Lett. 2018 PMID:30008936
089. Anti-Inflammatory Activity in Colon Models Is Derived from Δ9-Tetrahydrocannabinolic Acid That Interacts with Additional Compounds in Cannabis Extracts
Cannabis Cannabinoid Res. 2017 PMID:29082314
090. Effects of chemerin/CMKLR1 in obesity-induced hypertension and potential mechanism
Am J Transl Res. 2017 PMID:28670396
091. The atypical receptor CCRL2 is required for CXCR2-dependent neutrophil recruitment and tissue damage
Blood. 2017 PMID:28743719
092. Phoyunnanin E inhibits migration of non-small cell lung cancer cells via suppression of epithelial-to-mesenchymal transition and integrin αv and integrin β3
BMC Complement Altern Med. 2017 PMID:29284478
093. Escort cells generate a dynamic compartment for germline stem cell differentiation via combined Stat and Erk signalling
Development. 2017 PMID:28559239
094. Rho differentially regulates the Hippo pathway by modulating the interaction between Amot and Nf2 in the blastocyst
Development. 2017 PMID:28947533
095. RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos
Dev Biol. 2017 PMID:27836552
096. Myosin 1g Contributes to CD44 Adhesion Protein and Lipid Rafts Recycling and Controls CD44 Capping and Cell Migration in B Lymphocytes
Front Immunol. 2017 PMID:29321775
097. Progressive polarity loss and luminal collapse disrupt tissue organization in carcinoma
Genes Dev. 2017 PMID:28887414
098. Primary angle closure glaucoma (PACG) susceptibility gene PLEKHA7 encodes a novel Rac1/Cdc42 GAP that modulates cell migration and blood-aqueous barrier function
Hum Mol Genet. 2017 PMID:29016860
099. The desmoplakin–intermediate filament linkage regulates cell mechanics
Mol Biol Cell. 2017 PMID:28495795
100. Curcumin inhibits growth of human breast cancer cells through demethylation of DLC1 promoter
Mol Cell Biochem. 2017 PMID:27830358
101. Induction of cell cycle arrest by increasing GTP-RhoA levels via Taxol-induced microtubule polymerization in renal cell carcinoma
Mol Med Rep. 2017 PMID:28487984
102. Inhibitory effect of Angelica gigas on cold-induced RhoA activation in vascular cells
Mol Med Rep. 2017 PMID:28350093
103. 3D microniches reveal the importance of cell size and shape
Nat Commun. 2017 PMID:29213086
104. Role of Nicotinamide N-Methyltransferase in Dorsal Striatum in Cocaine Place Preference
Neuropsychopharmacology. 2017. PMID:28726800
105. EETs reduces LPS-induced hyperpermeability by targeting GRP78 mediated Src activation and subsequent Rho/ROCK signaling pathway
Oncotarget. 2017 PMID:28881620
106. inhibition of RhoA/Rho kinase pathway and smooth muscle contraction by hydrogen sulfide
Pharmacol Res Perspect. 2017 PMID:28971603
107. GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary
PLoS Genet. 2017 PMID:28072828
108. RhoA phosphorylation mediated by Rho/RhoA-associated kinase pathway improves the anti-freezing potentiality of murine hatched and diapaused blastocysts
Sci Rep. 2017 PMID:28751650
109. The role of the Nox4-derived ROS-mediated RhoA/Rho kinase pathway in rat hypertension induced by chronic intermittent hypoxia
Sleep Breath. 2017 PMID:28078487
110. Cannabidiol restores intestinal barrier dysfunction and inhibits the apoptotic process induced by Clostridium difficile toxin A in Caco-2 cells
United European Gastroenterol J. 2017 PMID:29238589
111. Mutations of Cystic Fibrosis Transmembrane Conductance Regulator Gene Cause a Monocyte-Selective Adhesion Deficiency
Am J Respir Crit Care Med. 2016 PMID:26694899
112. Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity
Cell Discov. 2016 PMID:27462462
113. Elongator controls cortical interneuron migration by regulating actomyosin dynamics
Cell Res. 2016 PMID:27670698
114. Curcumin inhibits LPA-induced invasion by attenuating RhoA/ROCK/MMPs pathway in MCF7 breast cancer cells
Clin Exp Med. 2016 PMID:25596714
115. Opposing effects of actin signaling and LFA-1 on establishing the affinity threshold for inducing effector T-cell responses in mice
Eur J Immunol. 2016 PMID:27188212
116. Gigantol Inhibits Epithelial to Mesenchymal Process in Human Lung Cancer Cells
Evidence-Based Complementary and Alternative Medicine. 2016 PMID:27651818
117. Thiopurine Prodrugs Mediate Immunosuppressive Effects by Interfering with Rac1 Protein Function
J Biol Chem. 2016 PMID:27189938
118. The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination with Cell Polarization During Ameloblast Differentiation.
J Bone Miner Res. 2016 PMID:27218883
119. VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells
J Cell Sci. 2016 PMID:27231093
120. Alpha 7 nicotinic receptor coupling to heterotrimeric G proteins modulates RhoA activation.cytoskeletal motility.and structural growth
J Neurochem. 2016 PMID:27167578
121. Converging.Synergistic Actions of Multiple Stress Hormones Mediate Enduring Memory Impairments after Acute Simultaneous Stresses
J Neurosci. 2016 PMID:27807170
122. RhoA determines lineage fate of mesenchymal stem cells by modulating CTGF-VEGF complex in extracellular matrix
Nat Commun. 2016 PMID:27126736
123. RASGRP1 deficiency causes immunodeficiency with impaired cytoskeletal dynamics
Nature Immunology,2016 PMID:27776107
124. TGFbeta induces binucleation/polyploidization in hepatocytes through a src-Dependent cytokinesis failure
PLoS One. 2016 PMID:27893804
125. p-Hydroxylcinnamaldehyde induces the differentiation of oesophageal carcinoma cells via the cAMP-RhoA-MAPK signalling pathway
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