NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Anti-Sar1GTP Mouse Monoclonal Antibody |
Cat. #: 26916 |
Size: 30 µL |
Gene Symbol: SAR1 |
Description: Anti-Sar1-GTP Mouse Monoclonal Antibody |
Background: Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER. |
Immunogen: Recombinant full length protein of active Sar1a. |
Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Sar1 GTPase) |
Published Applications: IP, IHC and IF – Click for Details |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-active Sar1 antibody recognizes active Sar1 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot: Sar1 Activation Assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti-active Sar1 monoclonal antibody (Cat. No. 26916). Immunoblot was with an anti-Sar1 rabbit polyclonal antibody (Cat. No. 21090). |
1. Mechanical strain stimulates COPII-dependent trafficking via Rac1 bioRxiv 2022 PMID: |
2. Increasing brain palmitoylation rescues behavior and neuropathology in Huntington disease mice Sci Adv. 202133789888 PMID: 33789888 |
3. cTAGE5/MEA6 plays a critical role in neuronal cellular components trafficking and brain development Proc Natl Acad Sci U S A. 2018 PMID: 30224460 |