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SARS-CoV-2 (Alpha) S protein RBD , hFc Tag

Recombinant SARS-CoV-2 S protein RBD(N501Y) protein with C-terminal human Fc tag
5/5

$445.00

Cat.#:  11343

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11343
Product Name:   SARS-CoV-2 (Alpha) S Protein RBD
Size :    10 µg, 50 µg and 100 µg
Synonyms:   SARS-CoV-2 B.1.1.7 (Alpha) Spike RBD Protein
Target:    S protein RBD
UNIPROT ID:    P0DTC2
Description:    Recombinant SARS-CoV-2 S protein RBD(N501Y) protein with C-terminal human Fc tag
Background:     SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide.The apparent molecular mass of RBD(N501Y)-hFc is approximately 55-70 kDa due to glycosylation.
Molecular Characterization:    S protein RBD(N501Y)(Arg319-Phe541) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD(N501Y), hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (Alpha) S protein RBD, hFc Tag (11343) can bind Human ACE2 Protein, mFc Tag 11195 in a linear range of 4.115-1000 ng/mL.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (Alpha) S protein RBD, hFc Tag (11343) can bind Anti-SARS-CoV-2 (CB6 biosimilar) mAb 28020 in a linear range of 1.00-97.66 ng/mL.

Figure 4. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (Alpha) S protein RBD, hFc Tag (11343) can bind Anti-SARS-CoV-2 RBD antibody (DM55), Rabbit mAb 28354 in a linear range of 0.244-15.625 ng/mL.
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