| Cat.#: 28056 |
| Product Name: Anti-Her2 (Trastuzumab)Monoclonal Antibody |
| Synonyms: ERBB2;D340;ER-2:neu;ER2;LN19;EU;GL;KR1 |
| Description: Anti-Her2 (trastuzumab biosimilar)Monoclonal Antibody |
| Background: Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| Applications: ELISA; Flow Cyt |
| Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
| Host Species: Humanized |
| Isotype: IgG1 |
| Purification: Purified from cell culture supernatant by affinity chromatography |
| Species Reactivity: Human Her2 |
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human Her2,His tagged protein 11214 can bind Anti-Her2 Neutralizing antibody (28056) in a linear range of 3.2-400 ng/ml. | 
Figure 2. ELISA plate pre-coated by 1 µg/mL (100 µl/well) Human Her2 Protein, hFc Tag (11461) can bind Anti-Her2 Neutralizing antibody (28056) in a linear range of 3.81-1730.10 ng/mL. In order to specifically detect 28056, mouse anti-human Fab-specific antibody was used as detection antibody. | 
Figure 3. Flow cytometry analysis with 15 µg/mL Anti-Her2 (trastuzumab) mAb (28056) on Expi293 cells transfected with Human Her2 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). | |
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