Cat.#: 28027 |
Product Name: Anti-CD27 (Varlilumab) Monoclonal Antibody |
Synonyms: CD27;TNFRSF7;S152;T14;Tp55 |
Description: Anti-CD27 (varlilumab ) Monoclonal Antibody |
Background: Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
Applications: ELISA; Flow Cyt |
Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
Host Species: Homo sapiens |
Isotype: IgG1 |
Purification: Purified from cell culture supernatant by affinity chromatography |
Species Reactivity: Human CD27 |
Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
 Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD27, hFc tagged protein (11285) can bind Anti-CD27 Neutralizing antibody in a linear range of 3.2-80.0 ng/ml. In order to specifically detect 28027, mouse anti-human Fab-specific antibody was used as detection antibody. |  Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD27, mFc-His tagged protein 11141 can bind Anti-CD27 Neutralizing antibody in a linear range of 0.64-16.0 ng/ml. |  Figure 3. HEK293 cell line transfected with irrelevant protein (B) and human CD27 protein (A) were surface stained with anti-CD27 neutralizing antibody 1µg/ml (varlilumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody. | |
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