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EGFR(N826S)

Anti-EGFR(N826S) Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26116

   Size:   100 μl

In Stock

          Product Description          

EGFR(N826S)

Cat. #:  26116
Gene Symbol:  EGFR/Her1/Neu
Description:   Anti-EGFR(N826S) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of N826S, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR(N826S), but not wild type EGFR protein from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing.

Western blot:  

Western blot analysis of recombinant EGFR(N826S) and wild type proteins.

Purified His-tagged EGFR(N826S) protein (702-987) (Cat. #10221) and corresponding wild type protein (702-987) (Cat. #10210) were blotted with anti-EGFR (N826S) mouse antibody (Cat. #26116).

Immunofluorescence:  

Immunofluorescence of cells expressing EGFR proteins with Anti-EGFR(N826S) antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(N826S) plasmid (right column), then fixed and stained with anti- EGFR(N826S) monoclonal antibody (Cat. #26116).

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