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Ran-GTP

Anti-Ran-GTP Monoclonal Antibody
5/5

$495.00

Cat.#:  26915

   Size:   30 μl

In Stock

            Product Description          

Ran-GTP

Cat. #:   26915
Size:  30 µL
Gene Symbol:  RAN
Description:   Anti-Ran-GTP Mouse Monoclonal Antibody
Background:   Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is involved in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis.
Immunogen:   Recombinant full length protein of active Ran.
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:   
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Ran-GTP monoclonal antibody recognizes active Ran of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze/thaw cycles
Immunoprecipitation/Western blot:  
Ran Activation Assay. Purified Ran proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-Ran-GTP monoclonal antibody (Cat. No. 26915). Immunoblot was with an anti-Ran polyclonal antibody.
          Publications          
01. CFDP1 regulates the stability of pericentric heterochromatin thereby affecting RAN GTPase activity and mitotic spindle formation
PLoS Biol. 2024  PMID: 38630655
02. Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation
J Mol Cell Biol. 2018  PMID: 29040603
03. OSBP-related protein 4L promotes phospholipase Cß3 translocation from the nucleus to the plasma membrane in Jurkat T-cells
J Biol Chem. 2018  PMID: 30237164
04. Initiation of DNA replication requires actin dynamics and formin activity
EMBO J. 2017  PMID: 28982779
05. SGK1 affects RAN/RANBP1/RANGAP1 via SP1 to play a critical role in pre-miRNA nuclear export: a new route of epigenomic regulation
Sci Rep. 2017  PMID: 28358001
06. Drosophila TIM Binds Importin a1, and Acts as an Adapter to Transport PER to the Nucleus
PLoS Genet., 2015  PMID: 25674790
07. Immunofluorescence methods in studies of the GTPase RAN and its effectors in interphase and in mitotic cells
Methods Mol Biol. 2014  PMID: 24470030
08. Activation of Ran GTPase by a Legionella Effector Promotes Microtubule Polymerization, Pathogen Vacuole Motility and Infection
PLoS Pathog., 2013  PMID: 24068924
09. IQGAP1 Protein Regulates Nuclear Localization of β-Catenin via Importin-β5 Protein in Wnt Signaling
J Biol Chem. 2013  PMID: 24196961
10. Calcium-calmodulin kinase I cooperatively regulates nucleocytoplasmic shuttling of CCTa by accessing a nuclear export signal
Mol Biol Cell. 2012  PMID: 22621903
11. NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3
Nucleic Acids Res. 2012  PMID: 22730302