Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT,PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).

Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of L858Q, human origin.

Tested applications: ELISA, WB, IF, IHC

Recommended dilutions:

ELISA:  1:1000-1:5000

WB:     1:500-1:1000

IHC:     1:50-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer: 

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes EGFR (L858Q),but not wild type EGFR protein from vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing.