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EML4 ALK

Anti-EML4 ALK Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26242

   Size:   100 μl

In Stock

          Product Description          
Anti-EML4 ALK Antibody
Cat. #:   26242
Gene Symbol:  EML4-ALK
Description:   Anti-EML4 ALK Mouse Monoclonal Antibody
Background:   EML4-ALK is a fusion-type protein kinase that can be found in many tumors, such as anaplastic large cell lymphoma, inflammatory myofibroblastic tumor, neuroblastoma and NSCLC, and so on. It is generated as a result of a small inversion within the short arm of human chromosome 2. EML4-ALK protein often promotes and maintains the malignant behavior of the cancer cells by activating the MAPK, PI3K/AKT and JAK/STAT3 pathways.
Immunogen:   A synthetic peptide from the internal region of EML4-ALK of human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:    ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes T41A mutant, but not wild type EML4-ALK of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant EML4-ALK and wild type proteins. Purified His-tagged EML4-ALK protein (lane 3), corresponding wild type EML4 protein (lane1) and wild type ALK protein (lane2) were blotted with Anti-EML4 ALK monoclonal antibody (Cat. #26242). Immunofluorescence:   Immunofluorescence of cells expressing EML4-ALK proteins with Anti-EML4 ALK antibody. HEK293T cells were transfected with pCDNA3-GFP-EML4 (WT) plasmid (left column) or pCDNA3-GFP-ALK (WT) plasmid (middle column) or pCDNA3-GFP-EML4-ALK plasmid (right column), then fixed and stained with Anti-EML4 ALK monoclonal antibody (Cat. #26242).
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