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Rab7-GTP

Anti-Rab7-GTP Monoclonal Antibody
5/5

$495.00

Cat.#:  26923

   Size:   30 μl

In Stock

            Product Description          

Rab7-GTP

Cat. #:   26923
Size:  30 µL
Gene Symbol:  RAB7
Description:   Anti-Rab7-GTP Mouse Monoclonal Antibody
Background:   Rab7 belongs to the Rab family of small GTPases. Rab7 is located in the late endosome, lysosome, cytoplasmic vesicle, phagosome and melanosome. It is involved in the late endocytic transport and contributes to the maturation of phagosomes.
Immunogen:   Recombinant full length protein of active Rab7
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:   
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Rab7-GTP monoclonal antibody recognizes active Rab7 proteins of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:  
Rab7 Activation Assay. Purified full-length Rab7 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-Rab7-GTP monoclonal antibody (Cat. No. 26923). Immunoblot was with an anti-Rab7 polyclonal antibody (Cat. No. 2 069).
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          Publications          
01. Celastrol directly binds with VAMP7 and RAB7 to inhibit autophagy and induce apoptosis in preadipocytes
Front Pharmacol. 2023   PMID:  36959859
02. Loss of small GTPase Rab7 activation in prion infection negatively affects a feedback loop regulating neuronal cholesterol metabolism
JBC. 2022  PMID: 
03. Celastrol Downmodulates Alpha-Synuclein-Specific T Cell Responses by Mediating Antigen Trafficking in Dendritic Cells
Front Immunol. 2022  PMID: 35309340
04. CD137-CD137L Aggravates Calcification of Vascular Smooth Muscle Cell and Vasculature of ApoE -/- Mice Via Rab7-Mediated Autophagy
J Cardiovasc Transl Res. 2022  PMID: 35763154
05. Restriction factor compendium for influenza A virus reveals a mechanism for evasion of autophagy
Nat Microbiol. 2021  PMID: 34556855
06. FCHSD2 controls oncogenic ERK1/2 signaling outcome by regulating endocytic trafficking
PLoS Biol. 2020  PMID: 32678845
07. Mechanisms underlying endolysosomal deficits mediated by the Parkinson’s disease-related kinase LRRK2
J Parkinsons Dis. 2020  PMID: 33044192
08. Discovery of a novel EGFR ligand DPBA that degrades EGFR and suppresses EGFR-positive NSCLC growth
Signal Transduct Target Ther. 2020  PMID: 33033232
09. Interferon-ß-induced miR-1 alleviates toxic protein accumulation by controlling autophagy
eLife. 2019  PMID: 31799933
10. The G2019S variant of leucine-rich repeat kinase 2 (LRRK2) alters endolysosomal trafficking by impairing the function of the GTPase RAB8A
J Biol Chem. 2019  PMID: 30709905
11. A SUMOylation-dependent switch of RAB7 governs intracellular life and pathogenesis of Salmonella Typhimurium
J Cell Sci., 2019  PMID: 30510112
12. Inhibition of TBC1D5 activates Rab7a and can enhance the function of the retromer cargo-selective complex
J Cell Sci. 2018  PMID: 29777037
13. Temozolomide-Perillyl alcohol conjugate impairs Mitophagy flux by inducing lysosomal dysfunction in non-small cell lung Cancer cells and sensitizes them to irradiation
J Exp Clin Cancer Res. 2018  PMID: 30326943
14. CASP4/caspase-11 promotes autophagosome formation in response to bacterial infection
Autophagy. 2018  PMID: 30165781
15. Rab7-a novel redox target that modulates inflammatory pain processing
Pain. 2017   PMID: 28394828