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Mouse B7-H3 Protein, hFc Tag

Recombinant mouse B7-H3 protein with C-terminal human Fc tag
5/5

$389.00

Cat.#:  12132

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     12132
Product Name:   Mouse B7-H3 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   6030411F23Rik;AU016588;B7h3;B7RP-2
Target:    B7-H3
UNIPROT ID:    Q8VE98
Description:    Recombinant mouse B7-H3 protein with C-terminal human Fc tag
Background:     Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells.[UniProtKB/Swiss-Prot Function]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 28.7 kDa after removal of the signal peptide.The apparent molecular mass of mB7-H3-hFc is approximately 55-70 kDa due to glycosylation.
Molecular Characterization:    Mouse B7-H3(Val29-Phe244) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Mouse B7-H3 Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Mouse B7-H3 Protein, hFc Tag  (12132) can bind anti-B7-H3 monoclonal antibody 28351 in a linear range of 3.2-400 ng/mL.
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