NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Cat. #: 26215 |
Size: 100 µL |
Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha |
Description: Anti-PIK3CA(D549N) Mouse Monoclonal Antibody |
Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer. |
Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of D549N, human origin. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
Concentration: 0.5 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes D549N mutant, but not wild type PIK3CA of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of recombinant PIK3CA(D549N) and wild type proteins. Purified His-tagged PIK3CA(D549N) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(D549N) monoclonal antibody (Cat. #26215). |
Immunofluorescence: Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(D549N) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(D549N) plasmid (right column), then fixed and stained with Anti-PIK3CA(D549N) monoclonal antibody (Cat. #26215). |