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CREBZF Rabbit pAb

Anti-CREBZF Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213320

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213320
Product Name:   Anti-CREBZF Rabbit Polyclonal Antibody
Synonyms:   ZF; SMILE
UNIPROT ID:   Q9NS37 (Gene Accession – NP_001034707 )
Background:    Host cell factor (HCF) is a cellular cofactor required for the activation of VP16 which expresses the herpes simplex virus immediate early gene. V16 binds to HCF through a 4-amino acid motif similar to the HCF binding domain of the basic leucine-zipper proteins Luman and Zhangfei (ZF). Luman activates promoters containing cAMP or unfolded protein response elements (UPRE). Zhangfei suppresses the transcriptional activity of Luman, but requires HCF binding which may target Luman and Zhangfei to a common location. Sequence analysis predicts that the deduced 272-amino acid Zhangfei protein has a negatively charged N terminus, a leucine zipper of 6 heptad leucine repeats separated by a conserved 6-amino acid spacer, a basic domain, and a bZIP region. It is also presumed that the N-terminal acidic region of Zhangfei is an activation domain.
Immunogen:    Synthetic peptide of human CREBZF
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 213320(CREBZF Antibody) at a dilution of 1/50(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 213320(Anti-CREBZF Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse brain tissue, PC3 cells;
Primary antibody: 213320(CREBZF Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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