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RELA Rabbit pAb

Anti-RELA Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216286

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216286
Product Name:   Anti-RELA Rabbit Polyclonal Antibody
Synonyms:   p65; CMCU; NFKB3; AIF3BL3
UNIPROT ID:   Q04206 (Gene Accession – BC014095 )
Background:    NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human RELA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Metabolism, Neuroscience, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 216286(RELA Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 216286(Anti-RELA Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 216286(Anti-RELA Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D220135(Anti-RELA Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: MCF7, Jurkat, Mouse liver tissue, Hela cell lysates;
Primary antibody: 216286(RELA Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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