Email: info@neweastbio.com   |  Call: 6109452007

MCM7 Rabbit pAb

Anti-MCM7 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216642

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216642
Product Name:   Anti-MCM7 Rabbit Polyclonal Antibody
Synonyms:   MCM2, CDC47, P85MCM, P1CDC47, PNAS146, P1.1-MCM3
UNIPROT ID:   P33993 (Gene Accession – BC013375 )
Background:    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Immunogen:    Fusion protein of human MCM7
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 15-50;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 216642(MCM7 Antibody) at a dilution of 1/15(Cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 216642(Anti-MCM7 Antibody) at dilution 1/15.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 216642(Anti-MCM7 Antibody) at a dilution of 1/15.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D220965(Anti-MCM7 Antibody) at dilution 1/15.

Gel: 10%SDS-PAGE, Lysate: 50 μg;
Lane: Jurkat cells;
Primary antibody: 216642(MCM7 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
          Publications