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AXIN2 Rabbit pAb

Anti-AXIN2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217197

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217197
Product Name:   Anti-AXIN2 Rabbit Polyclonal Antibody
Synonyms:   AXIL; ODCRCS
UNIPROT ID:   Q9Y2T1 (Gene Accession – BC006295 )
Background:    The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
Immunogen:    Fusion protein of human AXIN2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer, Neuroscience, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217197(AXIN2 Antibody) at a dilution of 1/75(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217197(Anti-AXIN2 Antibody) at dilution 1/75.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 217197(Anti-AXIN2 Antibody) at a dilution of 1/75.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D221962(Anti-AXIN2 Antibody) at dilution 1/75.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Human ileum tissue lysate ;
Primary antibody: 217197(AXIN2 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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