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SMURF2 Rabbit pAb

Anti-SMURF2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220074

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220074
Product Name:   Anti-SMURF2 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q9HAU4 (Gene Accession – NP_073576 )
Background:    E3 ubiquitin-protein ligase SMURF2 is an enzyme that in humans is encoded by the SMURF2 gene. E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3.
Immunogen:    Synthetic peptide of human SMURF2
Applications:    ELISA, IHC
Recommended Dilutions:    IHC: 25-100; ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gasrtic cancer tissue using 220074(SMURF2 Antibody) at a dilution of 1/15(Cytoplasm and Nucleus ).

In comparision with the IHC on the left, the same paraffin-embedded Human gasrtic cancer tissue is first treated with the synthetic peptide and then with 220074(Anti-SMURF2 Antibody) at dilution 1/15.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220074(Anti-SMURF2 Antibody) at a dilution of 1/15.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D260910(Anti-SMURF2 Antibody) at dilution 1/15.
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