Email: info@neweastbio.com   |  Call: 6109452007

UBE2I Rabbit pAb

Anti-UBE2I Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221208

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221208
Product Name:   Anti-UBE2I Rabbit Polyclonal Antibody
Synonyms:   P18; UBC9; C358B7.1
UNIPROT ID:   P63279 (Gene Accession – NP_003336 )
Background:    The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Immunogen:    Synthetic peptide of human UBE2I
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Metabolism, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221208(UBE2I Antibody) at a dilution of 1/40(Nucleus and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221208(Anti-UBE2I Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: TM4, hela, 293T and Jurkat cell;
Primary antibody: 221208(UBE2I Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
          Publications