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Nucleophosmin (2D9) Mouse mAb

Anti-Nucleophosmin (2D9) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261011

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261011
Product Name:   Anti-Nucleophosmin (2D9) Mouse Monoclonal Antibody
Synonyms:   B23; NPM
UNIPROT ID:   P06748
Background:    Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
Immunogen:    Purified recombinant full length of human NPM1 protein expressed in E.coli.
Applications:    WB,ChIP
Recommended Dilutions:    WB: 1/500-1/1000 ChIP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    2D9-C3-E5
MW:    Calculated MW: 33 kDa; Observed MW: 38 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of NPM1 in Hela, Jurkat and 3T3 lysates using NPM1 antibody.
          Publications