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Hsp27 Rabbit mAb

Anti-Hsp27 Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N262368

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N262368
Product Name:   Anti-Hsp27 Rabbit Monoclonal Antibody
Synonyms:   HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
UNIPROT ID:   P04792
Background:    Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway.
Immunogen:    A synthetic peptide of human Hsp27
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R07-9G2
MW:    Calculated MW: 23 kDa; Observed MW: 27 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Hsp27 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Hsp27 in mouse muscle, mouse heart lysates using Hsp27 antibody.

Western blot analysis of Hsp27 in K562 lysates using Hsp27 antibody.
          Publications