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NELFE Rabbit mAb

Anti-NELFE Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N262593

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N262593
Product Name:   Anti-NELFE Rabbit Monoclonal Antibody
Synonyms:   RD; RDP; RDBP; D6S45; NELF-E
UNIPROT ID:   P18615
Background:    Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.
Immunogen:    Recombinant protein of human NELFe
Applications:    WB,IHC-P,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R02-7E5
MW:    Calculated MW: 43 kDa; Observed MW: 43 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NELFe antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of NELFe in C6 lysates using NELFE antibody.
          Publications