NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Anti-Rab4GTP Monoclonal Antibody |
Cat. #: 26921 |
Size: 30 µL |
Gene Symbol: RAB4 |
Description: Anti-Rab4-GTP Monoclonal Antibody |
Background: Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic. |
Immunogen: Recombinant full length protein of active Rab4A. |
Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Rab4 GTPase) |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-Rab4-GTP monoclonal antibody recognizes active Rab4 from vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot: Rab4A Activation Assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-Rab4-GTP monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S (lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel. |