$495.00
| Anti-Arf6GTP Monoclonal Antibody |
| Cat. #: 26918 |
| Size: 30 µL |
| Gene Symbol: ARF6 |
| Description: Anti-Arf6-GTP Monoclonal Antibody |
| Background: ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis. |
| Immunogen: Recombinant full length protein of active Arf6. |
| Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Arf6 GTPase) |
| Published Applications: IP, IHC and IF – Click for Details |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-Arf6-GTP monoclonal antibody recognizes active Arf6 from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot: |
| Arf6 activation assay.
Purified full-length Arf6 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-Arf6-GTP monoclonal antibody (Cat. No. 26918). Immunoblot was with an anti-Arf6 polyclonal antibody (Cat. No. 21032). |
