NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$239.00
Cdc42 Monoclonal Antibody
Cat. #: 26008 |
Gene Symbol: Cdc42 |
Description: Anti-Cdc42 Mouse Monoclonal Antibody |
Background: Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
Immunogen: Recombinant full length protein of Cdc42 |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA 1:100-1:5000, WB 1:100-1:2000 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Cdc42 antibody recognizes Cdc42 from vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of Cdc42 proteins. MEF whole-cell lysate was used for western blot with the monoclonal anti-Cdc42 antibody (cat. # 26008). |
Glucocorticoid-mediated induction of caveolin-1 disrupts cytoskeletal organization inhibits cell migration and re-epithelialization of non-healing woundsCommun Biol. 2021 PMID:34145387 Application: IP |