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Anti-ABL(Y253H) Mouse Monoclonal Antibody


Cat.#:  26154

   Size:   100 μl

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            Product Description          


Cat. #:  26154
Size:  100 µL
Gene Symbol:  ABL1; ABL; JTK7; bcr-abl; c-ABL; p150; v-abl
Description:   Anti-ABL(Y253H) Mouse Monoclonal Antibody
Background:   V-abl Abelson murine leukemia viral oncogene homolog 1 also known as ABL1, is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, stress response and cell migration. Deficient in Abl can cause severe disease such as leukemia.
Immunogen:   A synthetic peptide from the internal region of Abl which includes the mutation of Y253H, human origin.
Applications:   ELISA, WB, IHC, IF
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  recognizes Abl Y253H, but not wild type of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:  

Western blot analysis of recombinant Abl(Y253H) and wild type proteins. Purified His-tagged Abl(Y253H) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-ABL(Y253H) mouse monoclonal antibody (Cat. #26154).

Immunofluorescence of cells expressing Abl proteins with anti-Abl(Y253H) antibody.

HEK293T cells were transfected with pCDNA3-GFP-Abl (WT) plasmid (left column) or pCDNA3-GFP-Abl(Y253H) plasmid (right column), then fixed and stained with anti-ABL(Y253H) monoclonal antibody (Cat. #26154).

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