NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
ABL(Y253H)
Cat. #: 26154 |
Size: 100 µL |
Gene Symbol: ABL1; ABL; JTK7; bcr-abl; c-ABL; p150; v-abl |
Description: Anti-ABL(Y253H) Mouse Monoclonal Antibody |
Background: V-abl Abelson murine leukemia viral oncogene homolog 1 also known as ABL1, is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, stress response and cell migration. Deficient in Abl can cause severe disease such as leukemia. |
Immunogen: A synthetic peptide from the internal region of Abl which includes the mutation of Y253H, human origin. |
Applications: ELISA, WB, IHC, IF |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: recognizes Abl Y253H, but not wild type of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of recombinant Abl(Y253H) and wild type proteins. Purified His-tagged Abl(Y253H) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-ABL(Y253H) mouse monoclonal antibody (Cat. #26154). |
Immunofluorescence: HEK293T cells were transfected with pCDNA3-GFP-Abl (WT) plasmid (left column) or pCDNA3-GFP-Abl(Y253H) plasmid (right column), then fixed and stained with anti-ABL(Y253H) monoclonal antibody (Cat. #26154). |