Subtotal:
$239.00
$495.00
ALK(C1156Y)
| Cat. #: 26226 |
| Gene Symbol: ALK, CD246, NBLST3 |
| Description: Anti-ALK(C1156Y) Mouse Monoclonal Antibody |
| Background: Anaplastic lymphoma kinase (ALK) also known as CD246 (cluster of differentiation 246) is a tyrosine kinase receptor that plays an important role in the development of the brain and nervous systems. Deficiencies in ALK, which most commonly as a fusion with other genes, are indicated in cancers. |
| Immunogen: A synthetic peptide from the internal region of ALK which includes the mutation of C1156Y, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes C1156Y mutant, but not wild type ALK of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant ALK(C1156Y) and wild type proteins. Purified His-tagged ALK(C1156Y) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-ALK(C1156Y) monoclonal antibody (Cat. #26226).
Immunofluorescence:
Immunofluorescence of cells expressing ALK proteins with Anti-ALK(C1156Y) antibody.
HEK293T cells were transfected with pCDNA3-GFP-ALK (WT) plasmid (left column) or pCDNA3-GFP-ALK(C1156Y) plasmid (right column), then fixed and stained with Anti-ALK(C1156Y) monoclonal antibody (Cat. #26226).
