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AKT Rabbit mAb

Anti-AKT Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N261811

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261811
Product Name:   Anti-AKT Rabbit Monoclonal Antibody
Synonyms:   AKT1
UNIPROT ID:   P31749/P31751/Q9Y243
Background:    Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Immunogen:    Recombinant protein of human AKT1/2/3
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R04-8A4
MW:    Calculated MW: 56 kDa; Observed MW: 56 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat,Hamster
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Hypoxia Signal Transductionyyyyy Hypoxia-induced
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2/3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of AKT in 3T3, mouse lung lysates using AKT antibody.

Western blot analysis of AKT1/2/3 in Jurkat, rat Brain, C6, CHO-K1, Hela lysates using AKT antibody.
          Publications