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ATF6B Rabbit pAb

Anti-ATF6B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218054

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218054
Product Name:   Anti-ATF6B Rabbit Polyclonal Antibody
Synonyms:   G13; CREBL1; CREB-RP
UNIPROT ID:   Q99941 (Gene Accession – BC008394 )
Background:    The protein encoded by this gene is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human ATF6B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 218054(ATF6B Antibody) at a dilution of 1/30(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 218054(Anti-ATF6B Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Mouse liver tissue, Hela cell, Human testis tissue, Jurkat and HEPG2 cell lysates;
Primary antibody: 218054(ATF6B Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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