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B3GNT3 Rabbit pAb

Anti-B3GNT3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221603

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221603
Product Name:   Anti-B3GNT3 Rabbit Polyclonal Antibody
Synonyms:   TMEM3; B3GN-T3; B3GNT-3; HP10328; B3GAL-T8; beta3Gn-T3
UNIPROT ID:   Q9Y2A9 (Gene Accession – NP_055071 )
Background:    This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking.
Immunogen:    Synthetic peptide of human B3GNT3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221603(B3GNT3 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221603(Anti-B3GNT3 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 221603(Anti-B3GNT3 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D263258(Anti-B3GNT3 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and 293T cell lysates;
Primary antibody: 221603(B3GNT3 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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