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BAG1 Rabbit pAb

Anti-BAG1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216291

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216291
Product Name:   Anti-BAG1 Rabbit Polyclonal Antibody
Synonyms:   HAP; BAG-1; RAP46
UNIPROT ID:   Q99933 (Gene Accession – BC001936 )
Background:    The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.
Immunogen:    Fusion protein of human BAG1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 216291(BAG1 Antibody) at a dilution of 1/40(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 216291(Anti-BAG1 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 50 μg;
Lane 1-3: Hela cells, K562 cells, Raji cells;
Primary antibody: 216291(BAG1 Antibody) at dilution 1/750;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 seconds
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