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BCAS2 Rabbit pAb

Anti-BCAS2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217201

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217201
Product Name:   Anti-BCAS2 Rabbit Polyclonal Antibody
Synonyms:   DAM1; SPF27; Snt309
UNIPROT ID:   O75934 (Gene Accession – BC005285 )
Background:    BCAS2 (breast carcinoma amplified sequence 2), also designated DAM1 (DNA amplified in mammary carcinoma 1 protein) or spliceosome-associated SPF 27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. BCAS2 is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TR? (thyroid hormone receptor beta), ER† (estrogen receptor alpha), ER?, PR (progesterone receptor), and PPAR? (Peroxisome proliferator-activated receptor gamma). BCAS2 functions as an ER coactivator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer.
Immunogen:    Fusion protein of human BCAS2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 217201(BCAS2 Antibody) at a dilution of 1/50(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 217201(Anti-BCAS2 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 217201(Anti-BCAS2 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D221974(Anti-BCAS2 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A172 cells, 231 cells;
Primary antibody: 217201(BCAS2 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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