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BCAS4 Rabbit pAb

Anti-BCAS4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220400

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220400
Product Name:   Anti-BCAS4 Rabbit Polyclonal Antibody
Synonyms:   CNOL
UNIPROT ID:   Q8TDM0 (Gene Accession – NP_060313 )
Background:    The gene encoding BCAS4 (Breast carcinoma-amplified sequence 4), a 211 amino acid protein, is found in a region on chromosome 20 that is frequently amplified in human breast cancer. This 20q13 locus amplification is found in 12%?39% of primary breast tumors, which correlates with a 17q23 locus amplification that is found in 20% of primary breast tumors. The amplification and translocation between the BCAS4 gene and the BCAS3 gene, with a 17q23 locus, results in a fusion transcript that is overexpressed in MCF-7 cells. Also, deletion of chromosomal region 20q13.13-q13.2 and resultant deletion of BCAS4, as well as three other genes, is the cause of Okihiro syndrome, a disease characterized by ocular and upper limb anomalies. BCAS4 is normally expressed in thymus, kidney, spleen, placenta and brain. There are three isoforms of BCAS4 which are produced as a result of alternative splicing events.
Immunogen:    Synthetic peptide of human BCAS4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastic cancer tissue using 220400(BCAS4 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastic cancer tissue is first treated with the synthetic peptide and then with 220400(Anti-BCAS4 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 220400(Anti-BCAS4 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D261480(Anti-BCAS4 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: K562 and Hela cell lysates, Human left kidney tissue and fetal intestines tissue lysates;
Primary antibody: 220400(BCAS4 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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