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BCL6B Rabbit pAb

Anti-BCL6B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218356

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218356
Product Name:   Anti-BCL6B Rabbit Polyclonal Antibody
Synonyms:   BAZF; ZNF62; ZBTB28
UNIPROT ID:   Q8N143 (Gene Accession – BC059404 )
Background:    Bcl-6, a transcriptional repressor, binds Stat recognition-like DNA elements and influences germinal center development and cell differentiation. Additionally, Bcl-6 negatively regulates NF?B expression, thereby inhibiting NF?B-mediated cellular functions. Bcl-6b (B-cell CLL/lymphoma 6, member B), also known as ZNF62, BAZF or ZBTB28, is a 480 amino acid nuclear protein that contains one BTB (POZ) domain and five C2H2-type zinc fingers. Expressed ubiquitously with highest expression in placenta and heart, Bcl-6b associates with Bcl-6 and functions as a sequence-specific transcriptional repressor that is thought to be necessary for early B-cell development. The gene encoding Bcl-6b maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.
Immunogen:    Fusion protein of human BCL6B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218356(BCL6B Antibody) at a dilution of 1/30(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218356(Anti-BCL6B Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: A549 cell lysate;
Primary antibody: 218356(BCL6B Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
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