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CDK4 Rabbit pAb

Anti-CDK4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216409

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216409
Product Name:   Anti-CDK4 Rabbit Polyclonal Antibody
Synonyms:   CMM3; PSK-J3
UNIPROT ID:   P11802 (Gene Accession – BC003644 )
Background:    The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Immunogen:    Fusion protein of human CDK4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 216409(CDK4 Antibody) at a dilution of 1/50(Nucleus and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 216409(Anti-CDK4 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 20 μg;
Lane 1-2: 293T and Hela cell lysates;
Primary antibody: 216409(CDK4 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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