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CREB1 Rabbit pAb

Anti-CREB1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216447

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216447
Product Name:   Anti-CREB1 Rabbit Polyclonal Antibody
Synonyms:   CREB; CREB-1
UNIPROT ID:   P16220 (Gene Accession – BC010636 )
Background:    This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Immunogen:    Fusion protein of human CREB1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 216447(CREB1 Antibody) at a dilution of 1/70(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 216447(Anti-CREB1 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 216447(Anti-CREB1 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with fusion protein and then with D220483(Anti-CREB1 Antibody) at dilution 1/70.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T, LO2, A375 cell lysates;
Primary antibody: 216447(CREB1 Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 15 seconds
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