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EEF1D Rabbit pAb

Anti-EEF1D Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219166

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219166
Product Name:   Anti-EEF1D Rabbit Polyclonal Antibody
Synonyms:   EF1D; EF-1D; FP1047
UNIPROT ID:   P29692 (Gene Accession – BC007847 )
Background:    This gene encodes a subunit of the elongation factor-1 comple,x which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.
Immunogen:    Fusion protein of human EEF1D
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 219166(EEF1D Antibody) at a dilution of 1/65(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 219166(Anti-EEF1D Antibody) at dilution 1/65.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 219166(Anti-EEF1D Antibody) at a dilution of 1/65.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with fusion protein and then with D225955(Anti-EEF1D Antibody) at dilution 1/65.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: A549, A172 and HepG2 cell lysates;
Primary antibody: 219166(EEF1D Antibody) at dilution 1/950;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 seconds
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