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EEF1E1 Rabbit pAb

Anti-EEF1E1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218161

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218161
Product Name:   Anti-EEF1E1 Rabbit Polyclonal Antibody
Synonyms:   P18; AIMP3
UNIPROT ID:   O43324 (Gene Accession – BC005291 )
Background:    This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218161(EEF1E1 Antibody) at a dilution of 1/35(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218161(Anti-EEF1E1 Antibody) at dilution 1/35.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 218161(Anti-EEF1E1 Antibody) at a dilution of 1/35.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D223851(Anti-EEF1E1 Antibody) at dilution 1/35.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: A549 cell, Jurkat cell, Human testis tissue, 293T cell, Hepg2 cell and A431 cell;
Primary antibody: 218161(EEF1E1 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 seconds
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