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EIF2S1 Rabbit pAb

Anti-EIF2S1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221937

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221937
Product Name:   Anti-EIF2S1 Rabbit Polyclonal Antibody
Synonyms:   EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
UNIPROT ID:   P05198 (Gene Accession – NP_004085 )
Background:    The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
Immunogen:    Synthetic peptide of human EIF2S1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 221937(EIF2S1 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 221937(Anti-EIF2S1 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 221937(Anti-EIF2S1 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D263768(Anti-EIF2S1 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: A549 cell, Human placenta tissue, A172 cell, NIH/3T3 cell, Jurkat cell, Hela cell lysates;
Primary antibody: 221937(EIF2S1 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
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