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ESR2 Rabbit pAb

Anti-ESR2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219764

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219764
Product Name:   Anti-ESR2 Rabbit Polyclonal Antibody
Synonyms:   Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
UNIPROT ID:   Q92731 (Gene Accession – NP_001428 )
Background:    This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.
Immunogen:    Synthetic peptide of human ESR2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Metabolism, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 219764(ESR2 Antibody) at a dilution of 1/20(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 219764(Anti-ESR2 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: 231, K562, PC-3, A172 and A549 cell lysates;
Primary antibody: 219764(ESR2 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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