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H2BC1 Rabbit pAb

Anti-H2BC1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221891

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221891
Product Name:   Anti-H2BC1 Rabbit Polyclonal Antibody
Synonyms:   STBP; TH2B; H2BFU; TSH2B; hTSH2B; TSH2B.1; HIST1H2BA; bA317E16.3
UNIPROT ID:   Q96A08 (Gene Accession – NP_733759 )
Background:    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]
Immunogen:    Synthetic peptide of human H2BC1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221891(H2BC1 Antibody) at a dilution of 1/45(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221891(Anti-H2BC1 Antibody) at dilution 1/45.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 221891(Anti-H2BC1 Antibody) at a dilution of 1/45.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D263694(Anti-H2BC1 Antibody) at dilution 1/45.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Human prostate tissue, PC-3, A549 and TM4 cell lysates;
Primary antibody: 221891(H2BC1 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
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